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Ikerbasque Eusko Jaurlaritza


Job Vacancies

This job offer was closed at 2022-09-30

University of the Basque Country

PREDOCTORAL RESEARCHER

Research Field: Biological sciences 
Sub Research Field:  

Job Summary

Identification and development of monoclonal antibodies against a conserved region of SARS-CoV-2 and employment of antibody engineering approaches to transform them into potent neutralizers.

Job Description

Identification of broadly neutralizing mAbs targeting the MPER region of SARS-CoV-2. Monoclonal antibodies will be isolated using single B cell cloning techniques and expressed in mamalian 293F cells for their purification and biophysical characterization (binding kinetics and stability measurements). Neutralization potency and breadth of the isolated antibodies will be measured using molecularly cloned S-pseudotyped viruses.

Potentiation of the neutralizing capacity of anti-MPER mAbs through protein engineering. The structure of the five mAbs with the best biophysical and functional properties will be solved in complex with its peptide epitope by X-ray crystallography. These molecular details will be used to guide the design of antibodies with improved biological functions.

Development of a nucleic acid delivery platform based on LNP that encode a monoclonal antibody. Plasmid DNA and nucleoside-modified mRNA encoding for the lead engineered antibody will be designed and formulated in LNPs. In order to optimize the LNP-based non-viral vectors, formulation composition, inclusion of different ligands (peptides, polysaccharides, etc) and technological factors will be tested. Transfection efficiency and cell viability studies of the optimized DNA and mRNA vectors will be tested in vitro in different cell models.

Benefits

Comment/web site for additional job details

https://apps.lanbide.euskadi.net/apps/of_detalle_oferta_trabajo?LG=C&IDRG=162022010692&CTRG=1&ORGN=

FP7 / PEOPLE / Marie Curie Actions

Research Framework Programme/
Marie Curie Actions
No 
SESAM Agreement Number  

Job Details

Type of Contract Temporary 
Status Full-time 
Hours Per Week 35  
Company/Institute  
Country SPAIN 
State/Province  
City  

Organization/Institute Contact Data

Organization University of the Basque Country 
Organization/Institution Type Academic 
Faculty/Department/Research Lab  
Country SPAIN 
City Vitoria 
State/Province Alava 
Postal Code  
Street  
E-Mail edurne.rujas@ehu.eus 
Website  
Phone  
Mobile Phone  
Fax  

Application Details

Envisaged Job Starting Date 2022-12-30 
Application Deadline 2022-09-30 
How To Apply e-mail  

Additional Requirements

Skill

 
Specific Requirements

Degree in pharmacy, or Master in Molecular Biology and Biomedicine, or Master in Pharmacology, or Master in Biomedical Research, or any Master related to Health Sciences that has been accessed through the Degrees in Pharmacy, Biochemistry, Biotechnology, or Biology.